Table 1 Human primer sequences used for RT-PCR
Gene | Accession | Sense primer | Antisense primer | Product Size (bp) |
|---|---|---|---|---|
Nestin | NM_006617.1 | CAGCGTTGGAACAGAGGTTGG | TGGCACAGGTGTCTCAAGGGTAG | 389 |
BMP 4 | NM_130851.2 | GTCCTGCTAGGAGGCGCGAG | GTTCTCCAGATGTTCTTCG | 338 |
Rex-1 | NM_174900 | GCGTACGCAAATTAAAGTCCAGA | ATCCTAAACCAGCTCGCAGAAT | 282 |
Gata-2 | NM_001145661.1 | AGCCGGCACCTGTTGTGCAA | TGACTTCTCCTGCATGCACT | 243 |
TDGF | NM_003212.3 | GCCCGCTTCTCTTACAGTGTGATT | AGTACGTGCAGACGGTGGTAGTTCT | 497 |
Sox 2 | NM_003106.3 | CCCCCGGCGGCAATAGCA | TCGGCGCCGGGGAGATACAT | 447 |
Leptin | NM_000230.2 | TGTGCCCATCCAAAAAGTCC | GTTTGGAGGAGACTGACTGCG | 100 |
PPARγ | NM_015869.4 | GAGCACTTCACAAGAAATTACC | AATGCTGGAGAAATCAACTG | 151 |
ALP | NM_000478.4 | GGAAGGGTCAGTCAGGTT | GTGGGCCGCTCTAGGCACCAA | 500 |
Osteocalcin | NM_199173.4 | ATGAGAGCCCTCACACTCCTC | GCCGTAGAAGCGCCGATAGGC | 294 |
TRAIL | NM_003810.2 | TTGTTGATGAAAAGTGCTAGAAATA | ATGGTCCATGTCTATCAAGTGC | 150 |
GAPDH | NM_002046.4 | TGAAGGTCGGAGTCAACGGATT | CATGTGGGCCATGAGGTCCACCAC | 530 |