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Figure 1 | Cancer Cell International

Figure 1

From: The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway

Figure 1

Curcumin’s responsiveness of MCF-7 and MDA-MB-231 cells. (A) WST-1 assay of MCF-7 and MDA-MB-231 cells after curcumin treatment. The indicated breast cancer cells were treated with indicated concentrations of curcumin for 12 or 24 h as described in the text. Data represents the fluorescent changes (n = 3). The experiments were performed three times. (B) Clonogenic analysis of MCF-7 and MDA-MB-231 cells after curcumin treatment. The indicated breast cancer cells were treated with 40 μM curcumin for 3 or 6 h, trypsinized, and plated in duplicates at low density (2,000 per well in 6 well plate). After 7–12 days, formed colonies were stained with crystal violet. Clones in a given area were counted for each condition. Represented is the percentage of viable clones after curcumin treatment respective to untreated cells. Columns, mean of three independent determinations; bars, SD.

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