Figure 2

Analysis of Cip/Kips expression in response to curcumin in MCF-7 and MDA-MB-231 cells. (A) Analysis of p21 (1:1000), p27 (1:1000) and p57 (1:500) changes induced by indicated concentration of curcumin for 24 h in MCF-7 and MDA-MB-231 cells was took by western blotting. Also shown is a blot for β-actin as a loading control. The representative data of at least two independent experiments are shown. (B) Analysis of p21 (1:1000), p27 (1:1000) and p57 (1:500) changes induced by 40 μM curcumin for indicated time in MCF-7 and MDA-MB-231 cells was took by western blotting. Also shown is a blot for β-actin as a loading control. The representative data of at least two independent experiments are shown. Quantitative analysis of Cip/Kips protein expression using Quantity One software (Bio-Rad, USA). Graphs show quantification of Cip/Kips protein expression after normalizing the data to respective control group.