Figure 3

Analysis of SKP2 at both protein and transcriptional level in response to curcumin. (A) Analysis of (1:1000) changes induced by indicated curcumin for 24 h in MCF-7 and MDA-MB-231 cells was taken by western blotting. Also shown is a blot for GAPDH as a loading control. The representative data of at least two independent experiments are shown. (B) Analysis of SKP2 (1:1000) changes induced by 40 μM curcumin for indicated time in MCF-7 and MDA-MB-231 cells was took by western blotting. Also shown is a blot for GAPDH as a loading control. The representative data of at least two independent experiments are shown. Quantitative analysis of SKP2 protein expression using Quantity One software (Bio-Rad, USA). Graphs show quantification of SKP2 protein expression after normalizing the data to respective control group. (C) MCF-7 or MDA-MB-231 cells were treated with indicated concentration of curcumin for 24 h. The bar graph indicates the fold change of SKP2 mRNA level in curcumin treated cells compared with non-treated cells. One-way ANOVA was studied, followed by Student’s t test. Each bar represents the mean ± SD of three independent experiments. *,P <0.05; **,P <0.01, compared with control. RT-Q-PCR, quantitative RT-PCR.