Table 1 Primer sets for RT-qPCR analysis

β-actin

Forward:

5′-CATCCGTAAAGACCTCTATGCCAAC-3′

186

NM_007393

Reverse:

5′-ATGGAGCCACCGATCCACA-3′

Mmp2

Forward:

5′-AGGCAGTAGAGTAAGGGGATCG-3′

279

NM_008610.2

Reverse:

5′-TAGAAAGTGTTCAGGTATTGCACTG-3′

Mmp3

Forward:

5′-TGAAGCATTTGGGTTTCTCTACT-3′

134

NM_010809

Reverse:

5′-GATGCCTTCCTTGGATCTCTTT-3′

Mmp9

Forward:

5′-GCCCTGGAACTCACACGACA-3′

85

NM_013599

Reverse:

5′-TTGGAAACTCACACGCCAGAAG-3′

Mmp13

Forward:

5′-TCCCTGGAATTGGCAACAAAG-3′

120

NM_008607.2

Reverse:

5′-GCATGACTCTCACAATGCGATTAC-3′

Mmp14

Forward:

5′-TCTTCAAGGAGCGATGGTTCT-3′

182

NM_008608.3

Reverse:

5′-CAGGGAGGCTTCGTCAAACA-3′

Vim

Forward:

5′-GGACGTTTCCAAGCCTGACCTC-3′

198

NM_011701

Reverse:

5′-CCGGTACTCGTTTGACTCCTGC-3′

Cdh1

Forward:

5′- ATTGCAAGTTCCTGCCATCCTC -3′

145

NM_009864

Reverse:

5′-CACATTGTCCCGGGTATCATCA-3′

Cdh3

Forward:

5′-TCGTGAGGACGAGCAGTTTG-3′

130

NM_007665

Reverse:

5′-GCCATGGTCGTTGATGTCAG-3′

Snail

Forward:

5′-AGGACGCGTGTGTGGAGTTC-3′

235

NM_011427

Reverse:

5′-TGGGAGCTTTTGCCACTGTC-3′

Slug

Forward:

5′-CATTCGAACCCACACATTGCC-3′

112

NM_011415

Reverse:

5′-AGAGAAAGGCTTTTCCCCAGTG-3′

Twist1

Forward:

5′-GCCGGAGACCTAGATGTCATTG-3′

149

NM_011658

Reverse:

5′-ACGCCCTGATTCTTGTGAATTTG-3′

Twist2

Forward:

5′-GCAAGCCAGGACCCACC-3′

100

NM_007855

Reverse:

5′-GTCATGAGGAGCCACAAGGT-3′

Zeb1

Forward:

5′-GCTGGCAAGACAACGTGAAAG-3′

116

NM_011546

Reverse:

5′-AGGATAAATGACGGCGGTGT-3′

Zeb2

Forward:

5′-AGACTTCACAGATCGAGCCT-3′

146

NM_015753

Reverse:

5′-CCTCCTGGGATTGGCTTGTT-3′