Figure 2
Effect of NA-2 and TMZ alone and in combinations on U87 cells growth inhibition. Cells were treated NA-2, TMZ and their combinations for 24 hrs and MTT assay was performed. Significant increase in growth inhibition was observed when TMZ (0.1 mM) combine with 0.33 mM of NA-2. Each bar represents mean ± S.E.M of three independent experiments. One-way ANOVA reveals a significant difference between individual drug treated cells and the cells receiving combined treatment of TMZ/NA-2 (###P <0.001). Whereas significant difference between control and treatment groups is indicated by ***P <0.001. Row under the figure represents the coefficient of drug interaction (CDI) = AB/(A × B). According to the absorbance of each group, AB is the ratio of the combination groups to control group; A or B is the ratio of the single agent groups to control group. Therefore, CDI value greater than, equal to or less than 1 specifies that the drugs are antagonistic, additive or synergistic respectively. The results show that TMZ and NA-2 synergistically inhibit growth of U87 cells when given in combination of 0.1 mM and 0.33 mM respectively.