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Figure 3 | Cancer Cell International

Figure 3

From: N-(2-hydroxyphenyl)acetamide (NA-2) and Temozolomide synergistically induce apoptosis in human glioblastoma cell line U87

Figure 3

(A) Representative photomicrographs presenting increased apoptosis in cells exposed to temozolomide in combination with NA-2 revealed by TUNEL assay. U87 cells were treated with TMZ and NA-2 as single agent or in combination as indicated. After 24 hrs TUNEL method was used to detect apoptotic cells and photographs were taken under the microscope. Note that the dark brown stain is indicative of apoptosis and is strongest in cells treated with TMZ and NA-2 in combination. (B) Bar graph showing percentage of apoptotic cells by TUNEL assay and each bar represents mean ± S.E.M of three independent experiments. Significant difference between vehicle control and treated cells is indicated by **P <0.01 and ***P <0.001. Whereas the significant difference between the individual drugs NA-2 or TMZ and their combination treatment is indicated by $$$P <0.001, ###P <0.001 respectively.

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