Figure 4

Effect of NA-2, TMZ and their combination on Bcl-2, Bax and Caspase-3 expression. Representative bands for BCL-2, BAX and Caspase-3 detected by RT PCR in vehicle control and treated cells. The housekeeping gene β-actin was amplified as an internal control. Graphic illustration of Bcl-2, Bax, Bax/ Bcl-2 ratio and Caspase-3 gene after normalizing with their β-actin expression also shown. Combination treatment with NA-2 and TMZ induce marked changes in the expression of all three mentioned genes as compared to their individual treatment. Each bar represents mean ± S.E.M of three independent experiments. Significant difference between vehicle control and treated cells was indicated by *P < 0.05, **P <0.01, ***P <0.001. The significant difference between the individual drugs NA-2 and TMZ and their combination treatment is indicated by ^$$P <0.01, ^$$$P <0.001 and ^###P <0.001 respectively.