M. hyorhinis binds to gastric cancer cell MGC803 in a time and dose-dependent manner. (A) M. hyorhinis binds to MGC803 cells in a time- and dose-dependent manner. The cells were exposed to 104, 105 CCU (color changing units)/mL of M. hyorhinis for 24 hours, or 105 CCU/mL of M. hyorhinis for 0 (unexposed), 12 and 24 hours. DAPI staining was performed at indicated time points. Titer of M. hyorhinis was quantified as color-change-units (CCU) per milliliter. One CCU equals to one organism of mycoplasma. Unless specified, we used 105CCU/mL M. hyorhinis to infect cells. By normalizing to the amount of cells to be infected, the multiplicity of infection (MOI) was 0.1 for 104CCU/mL and 1 for 105CCU/mL. (B) p37 binds to MGC803 cells. The cells were treated with 300 pmol GST-p37 for 24 hours. Immunofluorescence assay with PD4 antibody was performed after 24 hours. GST was used as negative control.