Figure 2
Morphological changes assayed in SP2/0 cells exposed to the indicated concentrations of hypericin at 24 h after applying 11.28 J/cm2irradiation dose. (A) Light microscopy analysis of morphological alternations in SP2/0 cells. (a) Untreated control cells. (b), (c) and (d) Cells treated with hypericin at 0.025, 0.05 or 0.1μΜ. Bar = 10 μm (B) Apoptotic or necrotic nuclei of SP2/0 cells evaluated using fluorescence microscopy with Hoechst 33342 (blue) /PI (red) double staining. (a) Untreated control cells. (b), (c) and (d) Cells treated with hypericin at 0.025, 0.05 or 0.1μΜ. Arrow: apoptotic nuclei; Arrow head: necrotic nuclei. Bar = 10 μm. (C) TEM analysis of ultrastructural alternations of SP2/0 cells. (a) Untreated control cells. (b), (c) and (d) Cells treated with hypericin at 0.025, 0.05 or 0.1μΜ. Arrow: apoptotic bodies; Arrow head: swollen mitochondria. (D) The percentage of apoptotic or necrotic nuclei was analyzed in a total of 200 cells from five random areas.