Figure 2

The impact of DOX and PTX on P-gp expression and activity. (A) The concentration-dependent effect of DOX and PTX on mRNA level of MDR1. The cells were treated with drugs for 12 h. (B) The time-dependent effect of drugs on mRNA levels of MDR1 with 100 nM DOX/25 nM PTX treatment. (C) Rho-123 fluorescence ratio in MCF-7 cells with DOX and PTX treatments at different concentrations. (D) Rho-123 fluorescence ratio in MCF-7 cells with 100 nM DOX/25 nM PTX treatment for 0, 2, 4, 8, 12, 24, 36, 48 h. (E) MDR1 mRNA levels in MCF-7 cells with 8 h/36 h DOX and PTX treatment. *, p < 0.05; **, p < 0.01 (n = 3). (F) Rho-123 fluorescence ratio in MCF-7 cells with 8 h/36 h DOX and PTX treatment. *, p < 0.05 (n = 3).