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Figure 2 | Cancer Cell International

Figure 2

From: Patterns of cancer cell sphere formation in primary cultures of human oral tongue squamous cell carcinoma and neck nodes

Figure 2

Representative phase contrast images of spheres (black arrows) formed in suspension in primary cultures of OTSCC (a,b,e) and neck nodes (c,f) in growth factor supplemented medium in non-adhesive 0.1% agarose coated plastic ware and H&E staining of neck nodes (d,g). (a) created and survived for 3 weeks. Daughter spheres were not formed. (b) created within 1 week, survived for 3 weeks. Re-passaged for 2 generations. (c) spheres formed in primary cultures of negative neck nodes within 24hours. Daughter spheres were not formed. (d) H&E staining of negative neck node (e) spheres created within 24 hours and survived for several weeks. Re-passaged for 3 generations. (f) spheres formed in primary cultures of positive neck nodes within 24hours. Daughter spheres were not formed. (g) H&E staining of positive neck node. Red arrow pointing to malignant cells. Magnification 40x.

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