Figure 2From: Patterns of cancer cell sphere formation in primary cultures of human oral tongue squamous cell carcinoma and neck nodesRepresentative phase contrast images of spheres (black arrows) formed in suspension in primary cultures of OTSCC (a,b,e) and neck nodes (c,f) in growth factor supplemented medium in non-adhesive 0.1% agarose coated plastic ware and H&E staining of neck nodes (d,g). (a) created and survived for 3 weeks. Daughter spheres were not formed. (b) created within 1 week, survived for 3 weeks. Re-passaged for 2 generations. (c) spheres formed in primary cultures of negative neck nodes within 24hours. Daughter spheres were not formed. (d) H&E staining of negative neck node (e) spheres created within 24 hours and survived for several weeks. Re-passaged for 3 generations. (f) spheres formed in primary cultures of positive neck nodes within 24hours. Daughter spheres were not formed. (g) H&E staining of positive neck node. Red arrow pointing to malignant cells. Magnification 40x.Back to article page