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Table 2 Number and size of spheres or spheroids in primary cultures and MACS isolated CD44+ cell cultures

From: Patterns of cancer cell sphere formation in primary cultures of human oral tongue squamous cell carcinoma and neck nodes

  Primary cell spheres CD44+ cell spheres
Tissue type   Parent spheres Passage 1 Passage 2 Passage 3 Parent spheres Passage 1 Passage 2 Passage 3 Passage 4
H Duration Number Cells/sphere 3 weeks None None None Cluster of cells 10-15 None None None None
15-20
10-30
OTSCC (Neck Node -) Duration Number Cells/sphere 1 week 1 week 1 week None 1 week 1 week 1 week 1 week None
30-40 15-20 10-15 20-30 20-30 10-15 Up to 10
20-50 10-25 up to 10 15-25 10-15 up to 10 3-5
OTSCC (Neck Node+) Duration Number Cells/sphere 1 day 1 day 1 day 1 day 1 day 1 day 1 day 1 day None
40-50 30-40 15-20 10-15 30-40 30-40 15-25 Up to 10
50-100 50-100 20-50 Up to 10 20-30 15-25 10-15 3-5
  1. Cells plated at a density of 250/cm2 in 10mL of conditioned media in 0.1% agarose or agar coated non-adhesive culture flasks. Triplicate analyses were done from each sample type.