Table 2 Number and size of spheres or spheroids in primary cultures and MACS isolated CD44+ cell cultures
| Â | Primary cell spheres | CD44+ cell spheres | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Tissue type | Â | Parent spheres | Passage 1 | Passage 2 | Passage 3 | Parent spheres | Passage 1 | Passage 2 | Passage 3 | Passage 4 |
H | Duration Number Cells/sphere | 3 weeks | None | None | None | Cluster of cells 10-15 | None | None | None | None |
15-20 | ||||||||||
10-30 | ||||||||||
OTSCC (Neck Node -) | Duration Number Cells/sphere | 1 week | 1 week | 1 week | None | 1 week | 1 week | 1 week | 1 week | None |
30-40 | 15-20 | 10-15 | 20-30 | 20-30 | 10-15 | Up to 10 | ||||
20-50 | 10-25 | up to 10 | 15-25 | 10-15 | up to 10 | 3-5 | ||||
OTSCC (Neck Node+) | Duration Number Cells/sphere | 1 day | 1 day | 1 day | 1 day | 1 day | 1 day | 1 day | 1 day | None |
40-50 | 30-40 | 15-20 | 10-15 | 30-40 | 30-40 | 15-25 | Up to 10 | |||
50-100 | 50-100 | 20-50 | Up to 10 | 20-30 | 15-25 | 10-15 | 3-5 | |||