Figure 5
Expression of NPM1 was regulated via the estrogen receptor-α (ERα) signaling in the primary-cultured FIGO stages I endometrial adenocarcinoma cells. The NMP1 mRNA expression (A) and protein expression (B) in primary-cultured cells treated with DMSO, 1 μM E2, 100 nM ICI, 1 μM E2 and 100 nM ICI, 1 μM PPT, 1 μM PPT and 100 nM ICI, 1 μM DPN for 24 h, respectively, were detected by qRT-PCR and Western blotting. E2, 17β-estradiol; ICI, ICI 182 780; PPT, 4,4′,4″(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol; DPN, 2,3-bis-(4-ydroxyphenyl)–propionitrile. *P < 0.05, **P < 0.01 indicate significant differences from those treated with DMSO.