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Figure 1 | Cancer Cell International

Figure 1

From: EHMT2 inhibitor BIX-01294 induces apoptosis through PMAIP1-USP9X-MCL1 axis in human bladder cancer cells

Figure 1

BIX-01294 induces apoptosis in human bladder cancer cells. (A) The indicated cell lines were seeded in 96-well plates on the first day and treated with 1.25, 2.5, 5, 10 and 20 μmol/l of BIX-01294 on the second day. After treatment for 24 hours, live cell number was estimated using SRB assay for calculation of cell survival. Points: mean of four replicate determinations; bars: S.D. (B) T24 and 5637 cells were treated with indicated concentrations of BIX-01294 for 24 hours and harvested for Western blot analysis. Levels of protein expression were analyzed by using antibodies against CASP8, CASP9, CASP3, PARP1 and ACTB. (C) T24 and 5637 cells were treated with 10 μmol/l of BIX-01294 for various lengths of time. Both attached and suspended cells were harvested for Western blot analysis. Levels of protein expression were analyzed by using antibodies against CASP8, CASP3, PARP1 and ACTB. CF: cleaved form. (D) Forty-eight hours after control siRNA and EHMT2 siRNA transfection, cells were treated with 10 μmol/l of BIX-01294. Whole-cell protein lysates were harvested for Western blot analysis. Levels of protein expression were analyzed by using antibodies against EHMT2, CASP8, CASP9, CASP3, PARP1 and ACTB.

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