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Figure 3 | Cancer Cell International

Figure 3

From: Antihelminthic benzimidazoles potentiate navitoclax (ABT-263) activity by inducing Noxa-dependent apoptosis in non-small cell lung cancer (NSCLC) cell lines

Figure 3

Induction of Noxa mRNA and protein in H292 cells treated with albendazole and mebendazole. (A) H292 cells were treated with or without 10 μM albendazole or mebendazole for 1 day. Cell lysate was prepared and Noxa, Bim, Puma, Mcl-1, Bcl-xL, and HPRT mRNA was measured using a branched DNA assay. The expression of these genes was normalized to HPRT control and no treatment control. (B) H292 cells were treated with increasing concentrations (0–10 μl) of albendazole and mebendazole for 2 days. Cell lysate was prepared and resolved on a 12% SDS polyacrylamide gel and probed with anti-Bim, anti-Noxa, and anti-Mcl-1. Antibody against actin was used as loading control. Data shown are representative of two independent experiments.

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