Figure 4

S100A7 knockdown inhibits proliferation in ANCI-H520 cells. S100A7 knockdown is stable over several cell passages with corresponding persistent decrease in growth rate of lung cancer cells. A. Quantitative reverse-transcription polymerase chain reaction confirms decreased messenger RNA (mRNA) over several cell passages 1 through 7. B. Analysis of proliferation using the same cell passages 1 through 7 shows a stable reduction in the growth rate of NCI-H520 knockdown cells. Controls for the experiments are wild-type cells and vector controls (NT). Data are presented as mean ± standard error of the mean; n =3 performed in triplicate. *P < 0.05.