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Figure 7 | Cancer Cell International

Figure 7

From: The role of individual caspases in cell death induction by taxanes in breast cancer cells

Figure 7

Effect of paclitaxel and SB- T- 1216 on cytochrome c release from mitochondria in SK -BR- 3 and MCF -7 cells. (A) After 36 h of incubation with tested taxane (100 nM for SK-BR-3 cells and 300 nM for MCF-7 cells), the localization of cytochrome c within the cells was detected using confocal microscopy (see “Materials and methods”). Control cells were incubated without taxane. The localization of cytochrome c (green), mitochondria (red), nuclei (blue) and the merge of cytochrome c, mitochondria and nuclei are shown. The data shown were obtained in one representative experiment of three independent experiments. (B) After 0, 12, 24, 36, 48 and 60 h of incubation with tested taxane (100 nM for SK-BR-3 cells and 300 nM for MCF-7 cells), levels of cytochrome c in mitochondrial (M) and cytosolic (C) fractions were determined using western blot analysis and relevant antibodies (see “Materials and methods”). COX IV (integral mitochondrial protein) level was used to confirm proper fractionation. MEK levels were used to confirm equal protein loading of cytosolic fraction. The data shown were obtained in one representative experiment of three independent experiments.

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