EGF stimulates interaction of
E-cadherin with Arf6. (A) Effect of EGF on the tyrosine phosphorylation of E-cadherin under EGF (50 ng/mL) treatment for up to 30 min were determined by immunoprecipitation; GAPDH was used as a loading control. n = 3 for the experiments above. (B) Cells treated with EGF (50 ng/mL, 15 min) were immunoprecipitated with anti-Arf6 antibody, followed by immunoblotting for E-cadherin. The second top panel shows immunoprecipitated Arf6. (C) Co-immunoprecipitation of E-cadherin by activated Arf6 was determined. Expression of activated Arf6 in total cell lysates is shown in the bottom panels. n = 3 for all experiments. *: P < 0.05, **: P < 0.01, referring to the difference between cells treated with and those without EGF.