Silencing Arf6 reduces internalization of
-cadherin by EGF. (A&B) The expression of Arf6 after treatment with Arf6 siRNA. Total protein extracts from T47D (A) and MCF-7 (B) cells transfected with Arf6 siRNA or control siRNA (mock) were analyzed by immunoblotting for Arf6. GAPDH was used a loading control. (C&D) Expression of biotin-labeled E-cadherin was determined by internalization assays in the T47D (C) and MCF-7 (D) cells transfected with siRNA for Arf6. **: P < 0.01, referring to the difference between cells treated with and those without EGF. ##: P < 0.01, referring to the difference between cells transfected with Arf6 siRNA plus EGF and the cells transfected with control siRNA plus EGF.