Figure 5

Effect of Arf6 inactivation on E -cadherin location in breast cancer cells. (A) Representative micrographs of MCF-7 cells treated with EGF and stained for E-cadherin. Cells transfected with Arf6-T27N or empty vector (GFP, green) were grown under EGF (50 ng/mL) for 15 min and examined E-cadherin expression (red) by immunofluorescence assay. The arrow shows that cells transfected with GFP-Arf6-T27N showed weaker E-cadherin internalization than control cells (red) after EGF treatment. (B) Representative micrographs of T47D cells treated with EGF and stained for E-cadherin. Cells transfected with Arf6 siRNA were grown under EGF (50 ng/mL) for 15 min and examined E-cadherin expression (green) by immunofluorescence assay. The arrow shows that cells transfected with Arf6 siRNA showed weaker E-cadherin internalization than control cells after EGF treatment. Scale bar, 10 μm. Images are representative of at least 3 independent determinations.