Sch B reduces the proliferation and viability of glioma cells. A. U87 and U251 cells were treated with various concentrations of Sch B for 24, 48 and 72 h. Cell viability and IC50 values were measured using the MTT assay. B and C. U87 and U251 cells were treated with Sch B(4, 8, 16μmol/L ) for 48 h and were then allowed to form colonies in fresh medium for 14 days. Representative Giemsa staining pictures of the colonies were shown (B). The numbers of colonies of U87 and U251 cells (C) were counted. The data shown are the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 vs. the control group.