Figure 2From: Inhibition of the AKT pathway in cholangiocarcinoma by MK2206 reduces cellular viability via induction of apoptosis MK2206 specifically inhibits phosphorylation at Serine 473 in CCA cells, leading to induction of apoptosis. After 96 hours of MK2206 treatment at the indicated doses, CCLP-1 and SG231 cells were lysed and Western blot analysis was used to determine expression levels of phosphorylated AKT and total AKT in addition to apoptotic markers (Pro-Caspase 3, Pro-Caspase 9, and Survivin).Back to article page