| | |
7 day EC50 (nM)
|
Combination EC50 (shift of SN-38 EC50 in presence of Niraparib)
|
|---|
| | | | |
Niraparib
|
|---|
| |
Cell line
|
Niraparib
|
SN-38
|
125 nM
|
250 nM
|
1000 nM
|
|---|
|
MSI
|
DLD1
|
3560
|
3.63
|
0.992
|
1.67
|
1.68
|
|
HCT8
|
2130
|
3.60
|
2.07
|
1.54
|
1.35
|
|
HCT15
|
1685
|
3.77
|
3.47
|
3.44
|
1.73
|
|
HCT116
|
6915
|
0.180
|
0.125
|
0.155
|
0.0991
|
|
LOVO
|
2010
|
3.43
|
2.3
|
3.53
|
2.4
|
|
LS174T
|
1310
|
0.331
|
0.257
|
0.208
|
0.231
|
|
LS180
|
3730
|
0.474
|
0.399
|
0.395
|
0.389
|
|
LS411N
|
1875
|
3.57
|
1.21
|
1.11
|
0.729
|
|
RKO
|
1870
|
0.958
|
0.593
|
0.778
|
0.184
|
|
RKOE6
|
1270
|
0.730
|
0.426
|
0.422
|
0.464
|
|
SNUC2A
|
>10000
|
3.23
|
3.04
|
2.72
|
2.52
|
|
SW48
|
>10000
|
1.79
|
0.886
|
0.762
|
0.598
|
|
MSS
|
COLO205
|
1990
|
1.57
|
0.572
|
0.475
|
0.531
|
|
HT29
|
6880
|
4.29
|
3.07
|
3.64
|
1.75
|
|
NCI-H-508
|
>10000
|
3.63
|
1.70
|
3.31
|
1.41
|
|
SK-CO-1
|
1090
|
0.416
|
0.178
|
0.334
|
0.143
|
|
SW403
|
>10000
|
3.39
|
1.14
|
1.87
|
0.387
|
|
SW480
|
>10000
|
29.0
|
11.9
|
11.6
|
8.7
|
|
SW620
|
552
|
5.91
|
3.62
|
UR
|
UR
|
|
SW837
|
>10000
|
UR
|
UR
|
UR
|
UR
|
|
SW948
|
7195
|
6.67
|
3.85
|
4.47
|
2.80
|
|
SW1116
|
>10000
|
UR
|
UR
|
UR
|
UR
|
|
SW1417
|
>10000
|
27.7
|
29.2
|
28.3
|
31.0
|
|
SW1463
|
>10000
|
26.4
|
22.2
|
UR
|
UR
|
|
T84
|
1460
|
1.01
|
0.491
|
0.352
|
0.210
|
- 7 day EC50 data for niraparib alone, SN-38 alone, and the combination EC50 at 125, 250 and 1000 nM of niraparib in a panel of 25 MSI and MSS CRC cell lines . The niraparib EC50 values are the average of n = 2 from previous experiments. The SN-38 EC50 values reported are for n = 1 for the experiment done on that day. Values listed under 125, 250, and 1000nM niraparib are the EC50 values for SN-38 in the presence of 125, 250 or 1000 nM niraparib (combination EC50). EC50 data was calculated using the inflection point of the curve in a 4 parameter fit in SoftMax Pro 5.2. UR indicates that the data is unreportable due to an inadequate curve.
