Figure 4From: The association between Salt-inducible kinase 2 (SIK2) and gamma isoform of the regulatory subunit B55 of PP2A (B55gamma) contributes to the survival of glioma cells under glucose depletion through inhibiting the phosphorylation of S6K B55gamma binds with SIK2 and increases protein stability of SIK2. A. B55gamma interacts with SIK2 in Glioma cells. Co-Immunoprecipitation was performed using B55gamma antibody and mouse IgG as control. Immunoblotting with SIK2 antibody showed B55gamma associated with SIK2. β-Actin expression in 5% whole cell lysates were showed as loading controls. B. Overexpression of B55gamma in U251 and U138 cells upregulated SIK expression. Western blot were performed with an anti-SIK2 and anti-B55gamma antibodies of total cell extract. β-Actin was a loading control. C. U251V and U251B55gamma (upper) cells were treated with CHX at 100 ug/ml or MG-132 at 5 uM for 0, 2, 4 and 8 hrs followed by Western blotting assay to exam the protein expression level of SIK2. U138V and U138B55gamma (lower) were treated with CHX at 100 ug/ml or MG-132 at 5 uM for 0, 2, 4 and 8 hrs followed by Western blotting assay to exam the protein expression level of SIK2.Back to article page