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Figure 4 | Cancer Cell International

Figure 4

From: The association between Salt-inducible kinase 2 (SIK2) and gamma isoform of the regulatory subunit B55 of PP2A (B55gamma) contributes to the survival of glioma cells under glucose depletion through inhibiting the phosphorylation of S6K

Figure 4

B55gamma binds with SIK2 and increases protein stability of SIK2. A. B55gamma interacts with SIK2 in Glioma cells. Co-Immunoprecipitation was performed using B55gamma antibody and mouse IgG as control. Immunoblotting with SIK2 antibody showed B55gamma associated with SIK2. β-Actin expression in 5% whole cell lysates were showed as loading controls. B. Overexpression of B55gamma in U251 and U138 cells upregulated SIK expression. Western blot were performed with an anti-SIK2 and anti-B55gamma antibodies of total cell extract. β-Actin was a loading control. C. U251V and U251B55gamma (upper) cells were treated with CHX at 100 ug/ml or MG-132 at 5 uM for 0, 2, 4 and 8 hrs followed by Western blotting assay to exam the protein expression level of SIK2. U138V and U138B55gamma (lower) were treated with CHX at 100 ug/ml or MG-132 at 5 uM for 0, 2, 4 and 8 hrs followed by Western blotting assay to exam the protein expression level of SIK2.

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