Figure 3

Effect of galangin on TPA-induced activity and expression of MMP-2/MMP-9 in HepG2 cells. (A) Cells were treated with 70 nM TPA for 12 h in serum-free medium and then incubated in various concentrations of galangin (0, 1, 2.5, and 5 μM) for 24 h. The conditioned media were collected and MMP-2/MMP-9 activities were determined by gelatin zymography. (B) Cells were treated with 70 nM TPA for 12 h and incubated in various concentrations of galangin (0, 1, 2.5, and 5 μM) for 12 h. And then, the RNA samples were extracted and subjected to a semi-quantitative RT-PCR for MMP-2 and MMP-9 with GADPH being an internal control.