Table 2 Purification scheme for LDH in cancerous breast samples
From: Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Fold purification | % yield |
|---|---|---|---|---|---|
Supernatant | 15 | 5.1 | 0.3 | - | - |
DEAE-Sephadex | 3.6 | 2.7 | 0.7 | 2.3 | 52 |
Blue Sepharose CL-6B | 0.2 | 0.73 | 3.6 | 12 | 14 |