Skip to main content

Table 4 Kinetic parameters of LDH in forward reaction from breast tumors (n =17) and normal tissues (n = 17)

From: Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues

 

Tumor

Normal

S 0.5 pyruvate (mM)

0.78 ± 0.07

0.63 ± 0.03

S 0.5 NADH (mM)

0.3 ± 0.01

0.33 ± 0.01

Vmax pyruvate (mU/mg protein)

4034 ± 348*

1747 ± 68

Vmax NADH (mU/mg protein)

2788 ± 111*

1370 ± 52

Ea (kcal/mol)

51 ± 4.1

41 ± 5.6

  1. Assays were conducted at 25°C and data are presented as means ± SEM, n = 3 independent determinations on each of 17 tumor and normal samples.*Significant difference in each row at p < 0.05.
Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us