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Table 4 Kinetic parameters of LDH in forward reaction from breast tumors (n =17) and normal tissues (n = 17)

From: Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues

  Tumor Normal
S 0.5 pyruvate (mM) 0.78 ± 0.07 0.63 ± 0.03
S 0.5 NADH (mM) 0.3 ± 0.01 0.33 ± 0.01
Vmax pyruvate (mU/mg protein) 4034 ± 348* 1747 ± 68
Vmax NADH (mU/mg protein) 2788 ± 111* 1370 ± 52
Ea (kcal/mol) 51 ± 4.1 41 ± 5.6
  1. Assays were conducted at 25°C and data are presented as means ± SEM, n = 3 independent determinations on each of 17 tumor and normal samples.*Significant difference in each row at p < 0.05.