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Table 5 Kinetic parameters of LDH in reverse reaction from breast tumors (n = 17) and normal tissues (n = 17)

From: Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues

  Tumor Normal
Km lactate (mM) 21.78 ± 1.07* 10.73 ± 0.54
Km NAD+ (mM) 0.99 ± 0.05* 0.50 ± 0.06
Vmax lactate (mU/mg protein) 630 ± 4.9* 602.2 ± 2.2
Vmax NAD+ (mU/mg protein) 1282 ± 71.24* 1237 ± 21.2
Ea (kcal/mol) 39.12 ± 4.6* 16.78 ± 1.7
  1. Assays were conducted at 25°C and data are presented as means ± SEM, n = 3 independent determinations on each of 17 tumor and normal samples. *Significant difference in each row at p < 0.05.