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Table 5 Kinetic parameters of LDH in reverse reaction from breast tumors (n = 17) and normal tissues (n = 17)

From: Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues

 

Tumor

Normal

Km lactate (mM)

21.78 ± 1.07*

10.73 ± 0.54

Km NAD+ (mM)

0.99 ± 0.05*

0.50 ± 0.06

Vmax lactate (mU/mg protein)

630 ± 4.9*

602.2 ± 2.2

Vmax NAD+ (mU/mg protein)

1282 ± 71.24*

1237 ± 21.2

Ea (kcal/mol)

39.12 ± 4.6*

16.78 ± 1.7

  1. Assays were conducted at 25°C and data are presented as means ± SEM, n = 3 independent determinations on each of 17 tumor and normal samples. *Significant difference in each row at p < 0.05.