Adenovirus-mediated co-expression of the TRAIL and HN genes inhibits growth and induces apoptosis in Marek’s disease tumor cell line MSB-1

Table 1 Primers used in the PCR amplification

Primer	Sequence
TRAIL-F	5′-GCCGCTACGATGCTGCCCGCG-3′
TRAIL-R	5′-ACCTGCAGGACACAGAATGGCAC-3′
HN-F	5′-ATGGACCGCGCGGTTAACAGAGTC-3′
HN-R	5′-TTAAACCTTATCATCCTTGAGG-3′
CMVtrF	5′-ATCTGGTACCACCATGCTGCCCGCGGGCGGG-3′
CMVtrM	5′-ATCATCTTTTTGATCAACTTTCCCAGGTGCCACTTCACTTGCCAGC
R	AGG-3′
CMVtrMF	5′-ACCTGGGAAAGTTGATCAAAAAGATGATGTCAAGAATCCTTCAGG
CMVtrR	AAAAC-3′
2AR	5′-TAGGCTCGAGCTAGCTTTGCTTCCTCCAG-3′
	5′-CATTGGGCCAGGATTCTCCTCGACGTCACCGCATGTTAGCAGA
	CTTCCTCTGCCCTCTCCACTGCCGCTTTGCTTCCTCCAGAGC-3′
2AF	5′-AGCGGCAGTGGAGAGGGCAGAGGAAGTCTGCTAACATGCGGT
CMVhnR	GACGTCGAGGAGAATCCTGGCCCAATGGACCGCGCGGTTAACAG-3′
CMVhnR	5′-TAGGCTCGAGTTAAACCTTATCATCCTT G-3′

The bold italics denote KpnI and XhoI enzyme cleavage sites; the italics denotes the site-directed mutagenesis of the enzyme cleavage sites PacI; the bold sequence denotes the 2A connecting peptide.

ISSN: 1475-2867