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Figure 1 | Cancer Cell International

Figure 1

From: Differential effects of HIF-α isoforms on apoptosis in renal carcinoma cell lines

Figure 1

Apoptosis in UV-treated 786-O cells with mutant VHL proteins correlates to HIF-2α levels. (A) 786-O cells stably expressing either an empty vector construct (control), wild-type VHLp30 (WT VHL), or various mutant VHLp30 proteins (as indicated) were grown to confluence and lysed. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for VHL, HIF-2α, and α-tubulin. VHL del 114-178 is a deletion of amino acid residues 114-178, whereas the rest of the VHL mutants are amino acid substitutions. (B) The 786-O cell lines in (A) were grown to confluence and treated with ultraviolet (UV) light and lysed one day later. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for poly (ADP-ribose) polymerase (PARP) and α-tubulin. The positions of the 116 kDa uncleaved PARP product and the 85 kDa cleaved fragment are indicated to the left. (C) Control 786-O cells and 786-O cells stably expressing wild-type VHLp19 (WT VHL) were grown to confluence and either left untreated or treated with ultraviolet (UV) light and lysed one day later. Western blots were performed for PARP and α-tubulin as in (B).

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