Figure 2

Apoptosis in UV-treated RCC10 cells with mutant VHL proteins correlates to HIF-2α levels. (A) RCC10 cells stably expressing either an empty vector construct (control), wild-type VHLp30 (WT VHL), or various mutant VHLp30 proteins [19] were grown to confluence and lysed. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for VHL, HIF-2α, and α-tubulin. (B) The RCC10 cell lines in (A) were grown to confluence and treated with UV light and lysed one day later. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for PARP, cleaved caspase-3, and α-tubulin. (C) Control RCC10 cells and RCC10 cells stably expressing wild-type VHLp30 (WT VHL) were grown to confluence and either left untreated or treated with ultraviolet (UV) light and lysed one day later. Western blots were performed for PARP, cleaved caspase-3, and α-tubulin as in (B).