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Figure 3 | Cancer Cell International

Figure 3

From: Differential effects of HIF-α isoforms on apoptosis in renal carcinoma cell lines

Figure 3

Reduction of HIF-2α levels leads to protection in UV-triggered apoptosis, but not for apoptosis caused by glucose and serum starvation in 786-O cells. (A) Parental 786-O or those either stably expressing wild-type VHLp19 or stably infected with a control vector (pSuperRetro) or a pool of two HIF-2α shRNAs vectors [21] were grown to confluence and lysed. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for HIF-2α, GLUT-1, VHL, and α-tubulin. (B) The 786-O cell lines described in (A) were grown to confluence and treated with UV light and lysed one day later. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for PARP, cleaved caspase-3, and α-tubulin. (C) The 786-O cell lines described in (A) were glucose starved for 24 hours as described in Methods. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for PARP, cleaved caspase-3, and α-tubulin. (D) The 786-O cell lines described in (A) were serum starved for 2 days as described in Methods. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for PARP, cleaved caspase-3, and α-tubulin.

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