Figure 5
From: Differential effects of HIF-α isoforms on apoptosis in renal carcinoma cell lines
Protection from apoptosis in cells with HIF-2α shRNA is seen for multiple glucose starvation time points. RCC10 cells stably expressing wild-type VHLp30, shRNA targeting luciferase (Luc shRNA), or a pool of two shRNAs targeting either HIF-1α or HIF-2α were glucose starved for 24, 36, or 48 hours, as indicated above the blot. Cell lysates were equally loaded and separated by SDS-PAGE. Western blots were performed for PARP, cleaved caspase-3, and α-tubulin.