is the YAP target gene in breast cancer MDA-MB-231 cells. The YAP shRNA and the lentiviral vector pLKO.1 stable cell lines were established in MDA-MB-231 cells. The cells were cultured to 90% confluence for extraction of RNA and protein. YAP mRNA and PBK mRNA were detected by PT-PCR (in A and B). The PBK protein level was detected by immunoblotting of the cell lysates (in C).