Combination of cyclophosphamide and double-stranded DNA demonstrates synergistic toxicity against established xenografts

Тable 1 Analysis of MCF-7 cells becoming apoptotic following culturing with fragmented exogenous human DNA and induced to apoptosis with TNFa

DNA fragment	Concentration, ng/mkl	Weight, mkg	Length, bp	*Cell number, 10** ⁶	Percentage of apoptotic cells
1,0	15.3	0.77
1,1	18.3	1.83
1,2	10.6	0.53	720	0.074	9.3
1,3	13.6	0.68	540	0.095	11.9
1,4	19.1	0.96	360	0.135	16.9
1,5	7.6	0.38	180	0.053	6.6
Lane 1	5.15			0.8	44.7
2,0	110.9	5.55
2,1	14.7	1.47
2,2	4.2	0.21	720	0.029	2.2
2,3	3.4	0.17	540	0.024	1.8
2,4	8.1	0.41	360	0.057	4.4
2,5	11.7	0.59	180	0.083	6.4
Lane 2		8.4		1.3	14.8

1,0, 2,0 – genomic DNA, 1,2-1,5, 2,2-2,5 – four most fast-migrating nucleosomal fragments, 1,1, 2,1 – larger fragments of apoptotic DNA.

ISSN: 1475-2867