Figure 1

CD70 protein is abundantly expressed on osteosarcoma cells. CD70 protein expression on the surface of (A) established osteosarcoma cell lines and EBV-transformed B cell lymphoma (EBV-B-LCL) cells and (B) patient-derived primary osteosarcoma cultures (Table 1) was analyzed by flow cytometry. Fold change of geoMFI of specific antibody stainings (bold solid line) compared to geoMFI of isotype control (light shade) is indicated in the representative FACS histogram plots. (C) CD70 protein expression (fold change geoMFI, calculated as geoMFI of specific antibody staining divided by geoMFI of the isotype control staining) on the surface of established osteosarcoma cell lines and patient-derived primary osteosarcoma cultures (geometric mean of three experiments), and of established Ewing sarcoma (geometric mean of two experiments), neuroblastoma (one experiment) and rhabdomyosarcoma (one experiment) cell lines. In addition to the indicated osteosarcoma cell lines, CD70 expression was detected on the Ewing sarcoma cell lines CADO-ES, ET10, STA-ET2.1, VH64, WE68 and the neuroblastoma line CHP126.