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Table 1 The primer sequences and reaction conditions

From: Interferon gamma polymorphisms and hepatitis B virus-related liver cirrhosis risk in a Chinese population

Locus

Primer

Annealing temperature

Method

Product size(bp)

+874 T/A

T allele primer: 5′-TTCTTACAACACAAATACAATACT-3′

61°C,56°C

SSP-PCR

AA,AT,TT:303

(rs2430561)

A allele primer: 5′-TTCTTACAACACAAATACAATACA-3′

 

common primer: 5′-TCCCATATAAACCTATAATATGCA-3′

 

inner reference F: 5′-CTTCAACACCCCAGCCTAGTAC-3′

Inner reference: 400

 

inner reference R: 5′-CCTCAGGGCAGCGAGACC-3′

+2109A/G (rs1861494)

F: 5′-GAGAGGTAAGGTGAGAGAGAACC-3′

55°C

RFLP-PCR (MspI)

AA:267

R: 5′-AGCAAAGAAGGTCTACAAACT-3′

AG:267 + 245 + 22

GG:245 + 22

  1. SSP-PCR,sequence-specific primer-polymerase chain reaction; RFLP-PCR, polymerase chain reaction-restriction fragment length polymorphism.