Locus | Primer | Annealing temperature | Method | Product size(bp) |
---|---|---|---|---|
+874 T/A | T allele primer: 5′-TTCTTACAACACAAATACAATACT-3′ | 61°C,56°C | SSP-PCR | AA,AT,TT:303 |
(rs2430561) | A allele primer: 5′-TTCTTACAACACAAATACAATACA-3′ | |||
common primer: 5′-TCCCATATAAACCTATAATATGCA-3′ | ||||
inner reference F: 5′-CTTCAACACCCCAGCCTAGTAC-3′ | Inner reference: 400 | |||
inner reference R: 5′-CCTCAGGGCAGCGAGACC-3′ | ||||
+2109A/G (rs1861494) | F: 5′-GAGAGGTAAGGTGAGAGAGAACC-3′ | 55°C | RFLP-PCR (MspI) | AA:267 |
R: 5′-AGCAAAGAAGGTCTACAAACT-3′ | AG:267 + 245 + 22 | |||
GG:245 + 22 |