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Figure 3 | Cancer Cell International

Figure 3

From: Apigenin, a dietary flavonoid, inhibits proliferation of human bladder cancer T-24 cells via blocking cell cycle progression and inducing apoptosis

Figure 3

Apigenin induced the loss of mitochondrial membrane potential and the release of cytochrome c in T-24 Cells. (A) Cultured cells were treated with indicated concentration (0, 20, and 30 μg/ml) of apigenin for 3 and 24 h. And then, mitochondrial membrane potential was measured by fluorescence microscope using JC-1 staining. (B) Cultured cells were treated with indicated concentration (0, 5, 10, 20, and 30 μg/ml) of apigenin for 24 h, and the expressions of cytochrome c in cytosol and mitochondria were assayed by Western blotting. The results were represented by using an ECL system and COX-4 was the internal control. The folds were compared with control. The results were obtained from at least three independent measurements.

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