Figure 4

Apigenin on the protein levels of proapoptotic proteins, antiapoptotic proteins, and the activation of the caspases cascade in T-24 cells. Cell were treated with various concentrations of apigenin for 24 h and 30 μg/ml apigenin for various periods of time (0, 1, 3, 6, and 12 h), and total cell lysates were prepared to detect (A) the protein levels of Bcl-xL, Bcl-2, Mcl-1, Bax, Bad, and Bak. (B) cleavage of caspase-3, caspase-7, caspase-9, and PARP by Western blotting analysis. The results were represented by using an ECL system and β-actin was the internal control. The folds were compared with control. The results were obtained from at least three independent measurements.