The effect of apigenin on cell cycle-related molecules in T-24 cells. (A, B, C) The levels of p53, p21, and p27 in T-24 cells were measured by ELISA kit. (D) The levels of p-p53, p53, p21, p27, Cyclin A, Cyclin B1, Cyclin E, CDK2, Cdc2, and Cdc25C were assessed by Western blotting assay. The results represented the average of three independent experiments ± S.D. *P < 0.05, **P < 0.01 compared with the untreated control; #P < 0.05, ##P <0.01 compared with the 0 h-treated time.