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Figure 3 | Cancer Cell International

Figure 3

From: Bim and VDAC1 are hierarchically essential for mitochondrial ATF2 mediated cell death

Figure 3

The interaction of VDAC1 and HK1 with ATF2 in response to apoptosis induction. (A) B16F10 cells stably transfected with scrambled or ATF2 shRNA were treated with PTX or PBS. Mitochondrial and Cytoplasmic extracts were subjected to an immunoblot analysis with the indicated antibodies. The positions of the molecular weight markers (in kDa) are noted on the right. (B) B16F10 cells stably transfected with ATF2 shRNA or ATF2T52A were crosslinked and lysed. VDAC1 was immunoprecipitated and subjected to an immunoblot analysis with the indicated antibodies. (C) B16F10 cells stably transfected with HK1 shRNA or VDAC1 shRNA were subjected to immunoprecipitated for ATF2 and then measured with immunoblot analysis using the indicated antibodies. (D) Cells stably transfected with HK1 shRNA, ATF2 shRNA, and VDAC1 shRNA were immunofluorescently stained with MitoTracker and anti-ATF2 antibodies after exposure with or without PTX and visualized using confocal microscopy (Olympus 1X81) (Scale bar: 20 μm).

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