Figure 1

Expression levels of miR-124 and talin 1 in prostate cancer. (A) Quantification of miR-124 and talin 1 expression in surgical specimens from 37 prostate cancer patients by real-time PCR. The relative expression level of miR-124 in cancerous tissue was reduced by more than half of that in adjacent tissue, while the expression level of talin 1 in cancerous tissue was elevated by twice that in adjacent tissue. (B) Correlation analysis of miR-124 and talin 1 expression in the specimens demonstrated an inverse relationship (r = −0.512). (C) Comparison of talin 1 levels in the metastasis group (14 cases) and non-metastasis group (23 cases) revealed a relatively higher level of talin 1 in the metastasis group by approximately 1.25-fold. (D) Levels of miR-124 were examined in the prostate cancer cell lines, which showed a remarkably reduced level of miR-124 compared with the normal epithelial cell line. (E) Talin 1 levels were examined in prostate cancer cell lines, which showed remarkably elevated expression compared with the normal epithelial cell line. (F) Increased expression level of talin 1 in cell lines as shown by western blotting. Data represent the means from three independent experiments (*p < 0.05).