Figure 4

MiR-124 impairs cell adhesion and motility by targeting talin 1 directly. Du145 cells were co-transfected with miR-124 mimics or NC RNA and p-TLN or p-CON plasmids. (A) Western blot analysis of talin 1. The decreased level of talin 1 induced by miR-124 mimics could be restored by forced expression of talin 1. (B) Attachment and (C) Transwell assays demonstrated functional effects in cells after transfection. p-TLN denotes the expression plasmid containing the full-length coding sequence of talin 1. p-CON denotes the control plasmid containing the non-sense sequence. Data represent the means from three independent experiments (*p < 0.05).