Figure 4

miR-378-5p down-regulates BRAF expression in human CRC cells. (A) Wild-type (WT) and mutant (Mut) of putative miR-378-5p targeting sequences in BRAF mRNA. Mutant sequences were shown in bold type. (B) miR-378-5p mimics inhibited wild-type but not mutant BRAF reporter activity in HEK 293 T cells. (C) miR-378-5p mimics down-regulated the endogenous BRAF mRNA levels in CRC cells. (D) miR-378-5p down-regulated the endogenous BRAF protein level in CRC cells. (E) Expression of BRAF was up-regulated in 47 CRC compared to adjacent non-tumor tissues. (F) A negative Spearman correlation between miR-378-5p and BRAF mRNA was found in 47 CRC tissues. *p <0.05, **p <0.01. NC, negative control oligonucleotides.