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Figure 1 | Cancer Cell International

Figure 1

From: Suramin inhibits cell proliferation in ovarian and cervical cancer by downregulating heparanase expression

Figure 1

Suramin decreases viability in HO-8910 PM ovarian cancer cells and Hela cervical cancer cells. HO-8910 PM and Hela cells were treated with Hpa inhibitor Suramin (50, 100, 200, 300, 400, 500 and 600 μg/ml). The cells (1 × 104) were incubated at these concentrations for 24, 48, 72 and 96 h at 37°C in a humid air atmosphere containing 5% CO2 in RPMI-1640 culture medium. Subsequently, 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) was added and the cells were incubated for 4 h; viability was assessed by measuring the OD at 490 nm, the value of the untreated control group (UCG) being taken as 100%. The assay showed that the HO-8910 PM and Hela cell vialibility decreased in a dose-dependent and time-dependent manner (a,c). After 48 h treatment with 300 μg/ml suramin, morphological changes became increasingly significant, and viable cells decreased markedly with dead cells floating and clumping in the culture media at 72 h (examined at 4-40X magnification using a Leica DMLB microscope) (b). Statistical analysis, the one-way ANOVA.* P < 0.05 24, 48, 72 and 96 h vs the UCG; 24 vs 72 h, or 96 h; 48 vs 72 h, or 96 h; 72 vs 96 h; P < 0.05 100, 200, 300, 400, 500,or 600 μg/ml vs the UCG; 100 vs 300, 400, 500,or 600 μg/ml, 200,vs 300, 400, 500 or 600 μg/ml, 300 vs 400, 500, or 600 μg/ml, 400 vs 500, 600 μg/ml, 500 vs 600 μg/ml.

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