Figure 3

Viability decrease in HO-8910 PM treated with 0, 50, 100 and 200 ug/ml Suramin. Suramin decreased viability and proliferation of HO-8910 PM cells at 50, 100, 200, 300, 400, 500 and 600 μg/ml (a,b,c,d). HO-8910 PM (1 × 10⁴) were incubated in the presence of these concentrations for 24, 48, 72 and 96 h at 37°C in a humid atmosphere containing 5% CO₂ and 95% air in RPMI-1640 culture medium. MTT was added and the cells were incubated for 4 h; viability was estimated by measuring the Optical density (OD) at 490 nm. The results represent the mean ± the standard deviation (SD) of all independent experiments performed in triplicate. Statistical analysis by one-way ANOVA. * P < 0.05 50 μg/ml 48 h vs 50 μg/ml 72 h; 50 μg/ml 72 h vs 50 μg/ml 96 h (b). ●P < 0.05: 100 μg/ml 48 h vs 100 μg/ml 72 h; 100 μg/ml 72 h vs 100 μg/ml 96 h (c). ♦ P < 0.05: 200 μg/ml 48 h vs 200 μg/ml 72 h; 200 μg/ml 72 h vs 200 μg/ml 96 h (d).