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Figure 7 | Cancer Cell International

Figure 7

From: Suramin inhibits cell proliferation in ovarian and cervical cancer by downregulating heparanase expression

Figure 7

Effect of suramin on Hpa protein and mRNA expression HO-8910 PM and the Hela cells. Slides of 48 h post-treatment with 300 μg/ml were selected for study. Both groups were tested in sextuplicate, and the experiment was repeated 3 times. Expression of Hpa protein was followed by SP immunohistochemical staining. Sections of colon cancer tissues positive for Hpa protein expression were used as the positive control, while antibody diluent replaced the antibody in the negative control. Compact brown or yellow immune particles of Hpa protein were present in the cytoplasm in the UCG, which distributed diffusely in all fields. The staining signals of Hpa protein in 300 μg/ml group decreased significantly in intensity as well as integration seen with a zoom lens of 40X by microscopy (b). HO-8910 PM cell Hpa mRNA expression was measured by an in situ hybridization kit. The slides were processed with an excessive amount RNA enzyme as the negative control. Sections of colon cancer tissues were used as the positive control. Compact blue hybridized granules of Hpa mRNA were present in the cytoplasm of the UCG, which distributed diffusely in all fields. The staining signals of Hpa mRNA in the 300 μg/ml group decreased significantly in intensity as well as integration (c). Real-time quantitative-PCR was used to determine Hpa mRNA expression in HeLa cells (a); its level in cells treated with 300 μg/ml suramin for 48 h was significantly lower than in untreated cells (300 μg/ml group 0.92 ± 0.87vs UCG 3.62 ± 2.80). Statistical analysis, chi-square test; *P <0.05. Hpa protein expression of 48 h post-treatment in the 300 μg/ml group vs the UCG; P < 0.05. Hpa mRNA expression of 48 h post-treatment in the 300 μg/ml group vs UCG.

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