Figure 2

Expression and cluster analysis of pediatric anaplastic histology Wilms tumor and normal tissues adjacent to cancer. Cluster analysis of the data was performed with gene clustering from the real-time PCR arrays. For gene expression quantification, we used the comparative Ct method. First, gene expression levels for each sample were normalized to the expression level of the housekeeping gene encoding glyceraldehyde 3-phosphate dehydrogenase within a given sample (−ΔCt). The relative expression of each gene was calculated using the equation: 10⁶ *Log₂ (−ΔCt). Gene expression between pediatric anaplastic histology Wilms tumor and normal tissues adjacent to cancer were analyzed using Multi Experiment View (MEV) cluster software.