Figure 2

HCT116 cells alter the expression of paracrine factors in AMSCs of co-culture model. P3 AMSCs were co-cultured with HCT116 cells in a transwell model. The characteristics and differentiate properties, and abilities of the expression of paracrine factors and cytokines in the co-cultured P3 AMSCs were examined. (A) The morphology and differentiation of original P3 AMSCs and AMSCs in the co-cultured model. AMSCs showed a normal MSC morphology and potency of osteogenic and adipogenic differentiation. The scale bar represents 20 μm. (B) Flow cytometry analysis for MSC-specific phenotype of AMSCs in the co-culture model. The HCT116 co-cultured P3 AMSCs retained a normal AMSC immunophenotype. (C-E) The alteration of expressions of indicated paracrine factors (C), cytokines (D) and EMT-associated genes in AMSCs was determined by a qRT-PCR assay. (F) Concentration of indicated paracrine factors and cytokines of the culture medium was ascertained by an ELISA. Data represented as mean ± SD from three independent triplicated experiments (N = 9). Compared to the control, *p < 0.05; **p < 0.01; ***p < 0.001.